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基本信息
載體類型: | 植物雙元表達載體 |
載體抗性: | Kan |
載體標簽: | / |
篩選標記: | 新霉素 |
啟動子: | Lac |
表達水平: | 高拷貝 |
載體大小: | 8740bp |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
5' 測序引物: | M13 Reverse:CAGGAAACAGCTATGAC |
3' 測序引物: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點信息
注意:
客戶在我公司選擇構(gòu)建載體服務(wù)項目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體描述
pCAMBIA系列載體是常用的植物表達雙元載體,載體的骨架是pUC18。其中 pCAMBIA1300, pCAMBIA2300均無gus報告基因,pCAMBIA系列載體均含有卡那霉素抗性基因。pCAMBIA2300特點:大腸桿菌篩選抗性卡那霉素,植物篩選抗性:新霉素。
載體序列
ORIGIN
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
2281 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
2341 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
2401 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
2461 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
2521 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
2581 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
2641 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
2701 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
2761 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
2821 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
2881 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
2941 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
3001 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
3061 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
3121 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
3181 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
3241 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
3301 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
3361 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
3421 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
3481 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
3541 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
3601 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
3661 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
3721 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
3781 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
3841 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
3901 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
3961 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
4021 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
4081 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4141 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4201 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
4261 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4321 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4381 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
4441 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
4501 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
4561 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
4621 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
4681 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
4741 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
4801 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
4861 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
4921 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata
4981 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata
5041 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt
5101 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa
5161 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt
5221 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc
5281 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc
5341 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc
5401 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag
5461 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag
5521 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac
5581 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc
5641 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta
5701 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct
5761 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc
5821 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg
5881 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca
5941 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt
6001 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag
6061 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat
6121 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga
6181 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt
6241 taatgtactg aattaacgcc gaattaattc gggggatctg gattttagta ctggattttg
6301 gttttaggaa ttagaaattt tattgataga agtattttac aaatacaaat acatactaag
6361 ggtttcttat atgctcaaca catgagcgaa accctatagg aaccctaatt cccttatctg
6421 ggaactactc acacattatt atggagaaac tcgagcttgt cgatcgactc tagctagagg
6481 atcgatccga accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga
6541 tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc
6601 cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca
6661 cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg
6721 gcaagcaggc atcgccatgt gtcacgacga gatcctcgcc gtcgggcatg cgcgccttga
6781 gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat
6841 cgacaagacc ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt
6901 cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg
6961 atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca
7021 atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc
7081 ccgtcgtggc cagccacgat agccgcgctg cctcgtcctg gagttcattc agggcaccgg
7141 acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg
7201 catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag
7261 cggccggaga acctgcgtgc aatccatctt gttcaatccc catggtcgat cgacagatct
7321 gcgaaagctc gagagagata gatttgtaga gagagactgg tgatttcagc gtgtcctctc
7381 caaatgaaat gaacttcctt atatagagga aggtcttgcg aaggatagtg ggattgtgcg
7441 tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga cgtggttgga
7501 acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg accactgtcg
7561 gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt gtaggtgcca
7621 ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg gaatccgagg
7681 aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc ttctgagact
7741 gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt cacatcaatc
7801 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
7861 gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct ttcctttatc
7921 gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga tgaagtgaca
7981 gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt gaaaagtctc
8041 aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga cgagagtgtc
8101 gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct ccccgcgcgt
8161 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
8221 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
8281 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
8341 tatgaccatg attacgaatt cgagctcggt acccggggat cctctagagt cgacctgcag
8401 gcatgcaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
8461 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
8521 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
8581 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
8641 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
8701 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg
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