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基本信息
載體類型: | 植物表達載體 |
載體抗性: | Kan |
載體標(biāo)簽: | C-EYFP |
篩選標(biāo)記: | / |
啟動子: | CaMV35S |
表達水平: | 高 |
載體大小: | 4058bp |
克隆方法: | 多克隆位點,限制性內(nèi)切酶 |
5' 測序引物: | 35S:5’-GACGCACAATCCCACTATCC-3’ |
3' 測序引物: | pEGFP N3: 5’-CGTCGCCGTCCAGCTCGACCAG-3’ |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點信息
注意:
客戶在我公司選擇構(gòu)建載體服務(wù)項目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體描述
植物過表達載體,帶EYFP報告基因,由pGreenII-62-SK改造而來,融合表達EYFP蛋白。
載體序列
ORIGIN
1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
541 ttggcaggat atattgtggt gtaacgttat cagcttggta cgtacccccc tactccaaaa
601 atgtcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
661 tttcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc gaaaggacag
721 tagaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gctatcattc
781 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
841 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgac atctccactg
901 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
961 gtcatttcat ttggagagga cagcccaagc tgagctccac cgcggtggcg gccgctctag
1021 aactagtgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
1081 cgagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
1141 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
1201 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
1261 caccctcgtg accaccttcg gctacggcct gcagtgcttc gcccgctacc ccgaccacat
1321 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
1381 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
1441 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
1501 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
1561 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
1621 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
1681 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
1741 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
1801 gtaaggtacc aattcggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc
1861 tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata
1921 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
1981 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
2041 cactagcctt gacaggatat attggcgggt aaactaagtc gctgtatgtg tttgtttgag
2101 atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
2161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
2221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
2281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
2341 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
2401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
2461 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
2521 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
2581 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
2641 ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
2701 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
2761 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
2821 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
2881 ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattagaaa aactcatcga
2941 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
3001 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
3061 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
3121 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
3181 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
3241 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa
3301 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
3361 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
3421 atgctgtttt ccctgggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
3481 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
3541 ctgtaacaac attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
3601 gcttcccata caatcggtag attgtcgcac ctgattgccc gacattatcg cgagcccatt
3661 tatacccata taaatcagca tccatgttgg aatttaatcg cggccttgag caagacgttt
3721 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
3781 ttgttcatga tgatatattt ttatcttgtg caatgtaaca tcagagattt tgagacacaa
3841 cgtggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc
3901 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
3961 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
4021 aggcgatccc catccaacag cccgccgtcg agcgggct
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