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基本信息

質(zhì)粒圖譜和多克隆位點信息

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載體描述 

pHAGE-CMV-MCS-IZsGreen為慢病毒表達質(zhì)粒；microRNA表達質(zhì)粒。

載體序列

ORIGIN

        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca

       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac

      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca

      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg

      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag

      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg

      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat

      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga

      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct

      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc

      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag

      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg

      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga

      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg

      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg

      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct

      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat

     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca

     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc

     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag

     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc

     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg

     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc

     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc

     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct

     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa

     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca

     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt

     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt

     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta

     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt

     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct

     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga

     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg

     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa

     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca

     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga

     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg

     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg

     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca

     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc

     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc

     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc

     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa

     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag

     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg

     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc catgttccat

     2821 gtttctttta ggtatatctt tggacttcct cccctgatcc ttgttctgtt gccagtagca

     2881 tcatctgatt gtgatattga aggtaaagat ggcaaacaat atgagagtgt tctaatggtc

     2941 agcatcgatc aattattgga cagcatgaaa gaaattggta gcaattgcct gaataatgaa

     3001 tttaactttt ttaaaagaca tatctgtgat gctaataagg aaggtatgtt tttattccgt

     3061 gctgctcgca agttgaggca atttcttaaa atgaatagca ctggtgattt tgatctccac

     3121 ttattaaaag tttcagaagg cacaacaata ctgttgaact gcactggcca ggttaaagga

     3181 agaaaaccag ctgccctggg tgaagcccaa ccaacaaaga gtttggaaga aaataaatct

     3241 ttaaaggaac agaaaaaact gaatgacttg tgtttcctaa agagactatt acaagagata

     3301 aaaacttgtt ggaataaaat tttgatgggc actaaagaac actaaaggat ccctcccccc

     3361 cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt

     3421 tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct

     3481 tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga

     3541 atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga

     3601 ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac

     3661 gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag

     3721 ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc

     3781 agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg

     3841 tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt

     3901 gaaaaacacg atgataatat ggccacacat atggcccagt ccaagcacgg cctgaccaag

     3961 gagatgacca tgaagtaccg catggagggc tgcgtggacg gccacaagtt cgtgatcacc

     4021 ggcgagggca tcggctaccc cttcaagggc aagcaggcca tcaacctgtg cgtggtggag

     4081 ggcggcccct tgcccttcgc cgaggacatc ttgtccgccg ccttcatgta cggcaaccgc

     4141 gtgttcaccg agtaccccca ggacatcgtc gactacttca agaactcctg ccccgccggc

     4201 tacacctggg accgctcctt cctgttcgag gacggcgccg tgtgcatctg caacgccgac

     4261 atcaccgtga gcgtggagga gaactgcatg taccacgagt ccaagttcta cggcgtgaac

     4321 ttccccgccg acggccccgt gatgaagaag atgaccgaca actgggagcc ctcctgcgag

     4381 aagatcatcc ccgtgcccaa gcagggcatc ttgaagggcg acgtgagcat gtacctgctg

     4441 ctgaaggacg gtggccgctt gcgctgccag ttcgacaccg tgtacaaggc caagtccgtg

     4501 ccccgcaaga tgcccgactg gcacttcatc cagcacaagc tgacccgcga ggaccgcagc

     4561 gacgccaaga accagaagtg gcacctgacc gagcacgcca tcgcctccgg ctccgccttg

     4621 ccctgaatcg atagatccta atcaacctct ggattacaaa atttgtgaaa gattgactgg

     4681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta

     4741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct

     4801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt

     4861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac

     4921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg

     4981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc

     5041 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg

     5101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct

     5161 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc

     5221 ctccccgcct gagatccttt aagaccaatg acttacaagg cagctgtaga tcttagccac

     5281 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg

     5341 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc

     5401 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg

     5461 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg

     5521 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca

     5581 aagaaatgaa tatcagagag tgagaggccc gggttaatta aggaaagggc tagatcattc

     5641 ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat

     5701 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt

     5761 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct

     5821 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc

     5881 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa

     5941 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg

     6001 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt

     6061 tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg

     6121 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac

     6181 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc

     6241 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa

     6301 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc

     6361 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt

     6421 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga

     6481 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa

     6541 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa

     6601 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa

     6661 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt

     6721 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt

     6781 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg

     6841 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt

     6901 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca

     6961 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac

     7021 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac

     7081 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct

     7141 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg

     7201 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca

     7261 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt

     7321 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta

     7381 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc

     7441 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc

     7501 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa

     7561 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag

     7621 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg

     7681 ttggccgatt cattaatgca gcaagctcat ggctgactaa ttttttttat ttatgcagag

     7741 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc

     7801 ctaggctttt gcaaaaagct ccccgtggca cgacaggttt cccgactgga aagcgggcag

     7861 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt

     7921 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa

     7981 cagctatgac atgattacga atttcacaaa taaagcattt ttttcactgc attctagttg

     8041 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc

     8101 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg

     8161 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat

     8221 ttgttaaata tgtactacaa acttagtagt

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