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基本信息

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意：

客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時(shí)，請(qǐng)自行提供母源載體。

如要使用本公司保存的母源載體時(shí)，本公司不能保證所有母源載體完全符合客戶預(yù)期，也不承擔(dān)由此產(chǎn)生的任何責(zé)任。  

載體描述

pCS2+載體能夠在多種細(xì)胞中進(jìn)行高水平的瞬表達(dá)。表達(dá)細(xì)胞：原核細(xì)胞、哺乳動(dòng)物、非洲爪蟾、鳥類、斑馬魚。pCS2+載體同時(shí)也能進(jìn)行體外轉(zhuǎn)錄和翻譯。本載體在pCS2+載體基礎(chǔ)上改造而來，增加了Flag蛋白標(biāo)簽，便于檢測(cè)和分離。

載體序列

ORIGIN

        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag

       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca tggattacaa ggatgacgac

      121 gataagtaat ctagaactat agtgagtcgt attacgtaga tccagacatg ataagataca

      181 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa

      241 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca

      301 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaattcg

      361 cggccgcggc gccaatgcat tgggcccggt acccagcttt tgttcccttt agtgagggtt

      421 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct

      481 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg

      541 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct

      601 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg

      661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc

      721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg

      781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct

      841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca

      901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct

      961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc

     1021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt

     1081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc

     1141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc

     1201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg

     1261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc

     1321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag

     1381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga

     1441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat

     1501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag

     1561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat

     1621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc

     1681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat

     1741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag

     1801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg

     1861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc

     1921 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca

     1981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg

     2041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc

     2101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta

     2161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc

     2221 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg

     2281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc

     2341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc

     2401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat

     2461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag

     2521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc

     2581 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt

     2641 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca

     2701 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta

     2761 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta

     2821 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg

     2881 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga

     2941 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg

     3001 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg

     3061 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc

     3121 cagtcgacca tagccaattc aatatggcgt atatggactc atgccaattc aatatggtgg

     3181 atctggacct gtgccaattc aatatggcgt atatggactc gtgccaattc aatatggtgg

     3241 atctggaccc cagccaattc aatatggcgg acttggcacc atgccaattc aatatggcgg

     3301 acttggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg

     3361 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca

     3421 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat

     3481 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg

     3541 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg

     3601 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc

     3661 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg

     3721 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac

     3781 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga

     3841 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg

     3901 caagtacatt actattggaa ggacgccagg gtacattggc agtactccca ttgacgtcaa

     3961 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg

     4021 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag

     4081 gtctatataa gcaatgctcg tttagggaac

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