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基本信息

載體類型: 

釀酒酵母表達(dá)載體 

載體抗性: 

Amp 

篩選標(biāo)記: 

URA3 

啟動(dòng)子: 

GPD 

高拷貝/低拷貝: 

低拷貝 

載體大小: 

5778 bp 

克隆方法: 

限制性內(nèi)切酶 

5' 測(cè)序引物: 

GPDpro-F: CGGTAGGTATTGATTGTAATTCTG 

3' 測(cè)序引物 

CYC1ter: GGTTGTCTAACTCCTTCCTT 

備注:

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質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意:

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如要使用本公司保存的母源載體時(shí),本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

載體序列

ORIGIN

    1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt

   61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata

  121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt

  181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa

  241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata

  301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct

  361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata

  421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact

  481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa

  541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg

  601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg

  661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt

  721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt

  781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg

  841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa

  901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt

  961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag

 1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt

 1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga

 1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt

 1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta

 1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg

 1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc

 1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt

 1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg

 1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg

 1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa

 1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa

 1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga

 1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg

 1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact

 1861 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac

 1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg

 1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg

 2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga

 2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc

 2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg

 2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc

 2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc

 2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt

 2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc

 2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc

 2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac

 2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact

 2641 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg

 2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt

 2761 aaccctcact aaagggaaca aaagctggag ctcagtttat cattatcaat actcgccatt

 2821 tcaaagaata cgtaaataat taatagtagt gattttccta actttattta gtcaaaaaat

 2881 tagcctttta attctgctgt aacccgtaca tgcccaaaat agggggcggg ttacacagaa

 2941 tatataacat cgtaggtgtc tgggtgaaca gtttattcct ggcatccact aaatataatg

 3001 gagcccgctt tttaagctgg catccagaaa aaaaaagaat cccagcacca aaatattgtt

 3061 ttcttcacca accatcagtt cataggtcca ttctcttagc gcaactacag agaacagggg

 3121 cacaaacagg caaaaaacgg gcacaacctc aatggagtga tgcaacctgc ctggagtaaa

 3181 tgatgacaca aggcaattga cccacgcatg tatctatctc attttcttac accttctatt

 3241 accttctgct ctctctgatt tggaaaaagc tgaaaaaaaa ggttgaaacc agttccctga

 3301 aattattccc ctacttgact aataagtata taaagacggt aggtattgat tgtaattctg

 3361 taaatctatt tcttaaactt cttaaattct acttttatag ttagtctttt ttttagtttt

 3421 aaaacaccag aacttagttt cgacggattc tagaactagt ggatcccccg ggctgcagga

 3481 attcgatatc aagcttatcg ataccgtcga cctcgagtca tgtaattagt tatgtcacgc

 3541 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct

 3601 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata

 3661 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa

 3721 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg gtacccaatt

 3781 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact

 3841 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct

 3901 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg

 3961 gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc

 4021 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt

 4081 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag

 4141 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt

 4201 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt

 4261 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt

 4321 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt

 4381 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc tgatgcggta

 4441 ttttctcctt acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt

 4501 aaattgaagc tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt

 4561 agttttgctg gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac

 4621 cctctacctt agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag

 4681 atcctgtaga gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt

 4741 catctaaacc cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca

 4801 tgtctctttg agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag

 4861 tacccttagt atattctcca gtagataggg agcccttgca tgacaattct gctaacatca

 4921 aaaggcctct aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac

 4981 ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac

 5041 ccgcagagta ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga

 5101 gtaaaaaatt gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa

 5161 aatcagtcaa gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa

 5221 ctaactccag taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct

 5281 tttcgtgcat gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt

 5341 ccttatatgt agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca

 5401 gttgggttaa gaatactggg caatttcatg tttcttcaac actacatatg cgtatatata

 5461 ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt accgaatcaa

 5521 aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt

 5581 gaaaagctgt ggtatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc

 5641 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat

 5701 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt

 5761 catcaccgaa acgcgcga

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