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基本信息
載體類型: | 轉(zhuǎn)座子載體 |
載體抗性: | Amp |
載體標(biāo)簽: | mCherry |
載體大小: | 3753bp |
克隆方法: | 多克隆位點(diǎn),限制性內(nèi)切酶 |
克隆菌株: | DH5a |
5' 測序引物: | pTolF: 5’-GAAAAAAAAGTACTTTTTACTCCTTAC-3’ |
3' 測序引物: | mCherryR:5’-CCTCGTAGGGGCGGCCCTCGCC-3’ |
備注: | 如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196 |
質(zhì)粒圖譜和多克隆位點(diǎn)信息
注意:
客戶在我公司選擇構(gòu)建載體服務(wù)項(xiàng)目時,請自行提供母源載體。
如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。
載體描述
由pTOL2-MCS改造而來,加入mCherry標(biāo)簽,可用于啟動子活性檢測。
載體序列
ORIGIN
1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
181 agtacttatt ttttggagat cacttagatc taactgcaga acgtacgaac ccgggaaacg
241 cgttctagag tcgacggtac cggatccggg cccgccacca tggtgagcaa gggcgaggag
301 gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg
361 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
421 accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc
481 cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac
541 ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
601 ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg
661 aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc
721 tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag
781 cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag
841 gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc
901 tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc
961 accggcggca tggacgagct gtacaagtag ctcgacaact tgtttattgc agcttataat
1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
1081 tctagttgtg gtttgtccaa actcatcaat gtatcttact cgagaaaagc ttaaacaaga
1141 atctctagtt ttctttcttg cttttacttt tacttcctta atactcaagt acaattttaa
1201 tggagtactt ttttactttt actcaagtaa gattctagcc agatactttt acttttaatt
1261 gagtaaaatt ttccctaagt acttgtactt tcacttgagt aaaatttttg agtacttttt
1321 acacctctga attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
1381 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
1441 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
1501 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
1561 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
1621 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
1681 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
1741 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
1801 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
1861 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
1921 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
1981 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
2041 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
2101 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
2161 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
2221 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
2281 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
2341 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
2401 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
2461 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
2521 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
2581 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
2641 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
2701 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
2761 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
2821 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
2881 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
2941 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
3001 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
3061 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
3121 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
3181 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
3241 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
3301 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
3361 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
3421 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
3481 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
3541 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
3601 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
3661 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
3721 tcagagcaga ttgtactgag agtgcaccat atg
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