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pET26b 構(gòu)建費(fèi)用200元/個起

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基本信息

載體類型: 

大腸桿菌表達(dá)載體 

載體抗性: 

Kan

載體標(biāo)簽: 

C-His 

表達(dá)水平: 

高拷貝 

載體大小: 

5360 bp 

啟動子: 

T7 

克隆方法: 

多克隆位點(diǎn),限制性內(nèi)切酶 

5' 測序引物: 

T75'-TAATACGACTCACTATAGGG-3' 

3' 測序引物 

T7 ter 

備注:

如有侵權(quán)請產(chǎn)權(quán)持有人盡快聯(lián)系法務(wù)15927229196

質(zhì)粒圖譜和多克隆位點(diǎn)信息

注意:

客戶在我公司選擇構(gòu)建載體服務(wù)項目時,請自行提供母源載體。

如要使用本公司保存的母源載體時,本公司不能保證所有母源載體完全符合客戶預(yù)期,也不承擔(dān)由此產(chǎn)生的任何責(zé)任。

載體序列

ORIGIN

        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa

       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt

      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt

      181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg

      241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc

      301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc

      361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc

      421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc

      481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg

      541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt

      601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg

      661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat

      721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt

      781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca

      841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa

      901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt

      961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg

     1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc

     1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta

     1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt

     1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg

     1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc

     1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac

     1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt

     1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa

     1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc

     1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt

     1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca

     1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag

     1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt

     1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta

     1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca

     1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg

     1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg

     2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct

     2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc

     2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga

     2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg

     2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg

     2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta

     2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat

     2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg

     2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta

     2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt

     2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca

     2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag

     2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt

     2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc

     2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt

     2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta

     3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac

     3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg

     3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg

     3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag

     3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac

     3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga

     3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt

     3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc

     3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc

     3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta

     3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat

     3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca

     3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct

     3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt

     3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct

     3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta

     3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag

     4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa

     4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt

     4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact

     4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga

     4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga

     4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca

     4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc

     4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg

     4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc

     4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat

     4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct

     4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg

     4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta

     4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg

     4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa

     4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca

     4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga

     5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc

     5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc

     5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc

     5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg

     5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac

     5341 agggcgcgtc ccattcgcca

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